Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Complement C3a Monoclonal Antibody (K13/16-5.7)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
Complement C3a Antibody (GAU 013-16-02) in WB
Western Blot was performed using Anti-Complement C3a Monoclonal Antibody (K13/16-5.7) (Product # GAU 013-16-02) and a 187 kDa band corresponding to Complement C3 was observed in HepG2 comparison to HEK-293 which is reported to be negative. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with Protein transport inhibitor(1X PTI for 4hr) (Lane 2), Hep G2 treated with LPS (10 ng/mL for 18 hr) followed by PTI(1X for 4hr) (Lane 3), HEK-293 (Lane 4), HEK-293 treated with PTI (1X for 4hr) (Lane 5) were electrophoresed ... View More
Product Details
GAU 013-16-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
K13/16-5.7
Immunogen
Human C3a.
Conjugate
Form
Liquid
Concentration
1.07 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_667457
Product Specific Information
GAU 013-16-02 detects Complement Componet C3a/C3a (desArg) in human samples. This antibody does not cross-react with C4a or C5a.
GAU 013-16-02 has been successfully used in ELISA and Western blot procedures. GAU 013-16-02 can be used as a capture antibody in sandwich ELISA with GAU 017-01-02 as detection antibody.
This antibody detects an epitope that is present on human C3, C3a, and C3a (desArg), but does not cross react with C4a or C5a.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; C3r1; Complement C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; prepro-C3
Gene Aliases: AHUS5; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p
UniProt ID: (Human) P01024
Entrez Gene ID: (Human) 718
positive regulation of type IIa hypersensitivity
positive regulation of protein phosphorylation
positive regulation of activation of membrane attack complex
tolerance induction
proteolysis
fatty acid metabolic process
inflammatory response
immune response
complement activation
complement activation, alternative pathway
complement activation, classical pathway
signal transduction
G-protein coupled receptor signaling pathway
blood coagulation
positive regulation of vascular endothelial growth factor production
positive regulation of glucose transport
regulation of triglyceride biosynthetic process
positive regulation of lipid storage
negative regulation of endopeptidase activity
regulation of complement activation
response to magnesium ion
response to estradiol
response to progesterone
response to estrogen
positive regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of developmental growth
regulation of immune response
response to glucocorticoid
positive regulation of ERK1 and ERK2 cascade
positive regulation of apoptotic cell clearance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support